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chromogenic reagent (3,3′-diaminobenzidine/dab) kits  (ZSGB Biotech)

 
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    Structured Review

    ZSGB Biotech chromogenic reagent (3,3′-diaminobenzidine/dab) kits
    Chromogenic Reagent (3,3′ Diaminobenzidine/Dab) Kits, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dab+chromogenic+reagent+kit/chromogenic+reagent++3+3++diaminobenzidine+dab++kits/pm40337977-48-3-13
    Average 90 stars, based on 1 article reviews
    chromogenic reagent (3,3′-diaminobenzidine/dab) kits - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities.
    Article Snippet: Complement factor I (Cfi) is a soluble serine protease which plays a crucial role in the modulation of complement cascades.. In the presence of substrate modulating cofactors (such as complement factor H, C4bp, CR1, etc), Cfi cleaves and inactivates C3b and C4b, thereby controlling the complement-mediated processes.. In this study, we sequenced and characterized Cfi gene from Cynoglossus Semilaevis (designated as CsCfi) for the first time.

    Membrane:

    Article Title: Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities.
    Article Snippet: Complement factor I (Cfi) is a soluble serine protease which plays a crucial role in the modulation of complement cascades.. In the presence of substrate modulating cofactors (such as complement factor H, C4bp, CR1, etc), Cfi cleaves and inactivates C3b and C4b, thereby controlling the complement-mediated processes.. In this study, we sequenced and characterized Cfi gene from Cynoglossus Semilaevis (designated as CsCfi) for the first time.

    Blocking Assay:

    Article Title: Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities.
    Article Snippet: Complement factor I (Cfi) is a soluble serine protease which plays a crucial role in the modulation of complement cascades.. In the presence of substrate modulating cofactors (such as complement factor H, C4bp, CR1, etc), Cfi cleaves and inactivates C3b and C4b, thereby controlling the complement-mediated processes.. In this study, we sequenced and characterized Cfi gene from Cynoglossus Semilaevis (designated as CsCfi) for the first time.

    Incubation:

    Article Title: Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities.
    Article Snippet: Complement factor I (Cfi) is a soluble serine protease which plays a crucial role in the modulation of complement cascades.. In the presence of substrate modulating cofactors (such as complement factor H, C4bp, CR1, etc), Cfi cleaves and inactivates C3b and C4b, thereby controlling the complement-mediated processes.. In this study, we sequenced and characterized Cfi gene from Cynoglossus Semilaevis (designated as CsCfi) for the first time.

    Article Title: Identification, expression profile and analysis of the antimicrobial activity of collectin 11 (CL-11, CL-K1), a novel complement-associated pattern recognition molecule, in half-smooth tongue sole (Cynoglossus semilaevis).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Saline:

    Article Title: Influence of MDR1 methylation on the curative effect of interventional embolism chemotherapy for cervical cancer
    Article Snippet: .. The first antibody (mouse anti-P-gp monoclonal antibody purchased from Abcam PLC [Cambridge, UK]) was added and put in a humidified box at 4°C for staying overnight, washed with Tris-buffered saline for three times, diaminobenzidine developing (DAB Chromogenic Reagent Kit by ZSGB-BIO, Beijing, People’s Republic of China), hematoxylin staining for 1 minute, dehydration and transparency, and finally sealed by neutral balsam. ..

    Staining:

    Article Title: Influence of MDR1 methylation on the curative effect of interventional embolism chemotherapy for cervical cancer
    Article Snippet: .. The first antibody (mouse anti-P-gp monoclonal antibody purchased from Abcam PLC [Cambridge, UK]) was added and put in a humidified box at 4°C for staying overnight, washed with Tris-buffered saline for three times, diaminobenzidine developing (DAB Chromogenic Reagent Kit by ZSGB-BIO, Beijing, People’s Republic of China), hematoxylin staining for 1 minute, dehydration and transparency, and finally sealed by neutral balsam. ..

    Article Title: ADP-ribosylation factor 6 promotes infectious bursal disease virus replication by affecting the internalization process via clathrin.
    Article Snippet: ADP-ribosylation factor 6 (ARF6) is a small G protein with extensive functions, including regulation of cellular membrane transport and viral infection.. Infectious bursal disease (IBD) is caused by infectious bursal disease virus (IBDV), which mainly invades the bursa of Fabricius and causes low immunity in poultry.. Our study demonstrated that IBDV infection could promote the expression of ARF6; however, the underlying mechanism remains unclear.

    Molecular Weight:

    Article Title: Effect of Berberine from Coptis chinensis on Apoptosis of Intestinal Epithelial Cells in a Mouse Model of Ulcerative Colitis: Role of Endoplasmic Reticulum Stress
    Article Snippet: .. The chemicals utilized in this study were as follows: dextran sulfate sodium (DSS; MP Biomedicals, LLC, Solon, OH, USA; lot number: BJ14745, molecular weight: 5000); berberine hydrochloride (BBR; Shanghai Yuanye Bio-technology Co., Ltd., Shanghai, China; lot number: Y18D8C50814, molecular weight: 371.81); glucose-regulated protein 78 (GRP78), caspase-12, and caspase-3 primary antibodies (Abcam, Cambridge, UK; lot numbers: GR309483-1, ab62484, and ab13847, respectively); anti-rabbit and anti-mouse secondary antibody (Beijing ZSGB-BIO, Beijing, China; lot numbers: K167722B and K175212C, respectively); proteinase K (Beijing Tiangen, Beijing, China; lot number: M2011); DAB chromogenic reagent kit (Beijing ZSGB-BIO, lot number: K167722A); TUNEL in situ apoptosis detection kit (Roche, Basel, Switzerland; lot number: 11906800); high-purity total RNA rapid extraction kit (Shanghai Generay, Shanghai, China; lot number: 1703G01); HiScript-II Q RT SuperMix for qPCR reverse transcription kit (Nanjing Vazyme, Nanjing, China; lot number: 7E092G6); and ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme Co.; lot number: 7E092H6). .. The following instruments were used in this study: a RM2235 rotary microtome (Leica, Wetzlar, Germany); TEC-2500 histopathology dryer (Changzhou Hao Silin Instrument Equipment Co., Ltd., Nanjing, China); BX43 microscope (Olympus, Tokyo, Japan); PYX-DHS500BS-II water jacket constant-temperature incubator (Shanghai Yuejin Medical Instrument Co., Ltd.); BCD-211KD3 refrigerator (TCL, Huizhou, China); C21-SDHC15K induction heater (Zhejiang Shaoxing Supor Life Electric Co., Ltd., Hangzhou, China); and 101-3 electric heating constant-temperature dryer (Shanghai Jinping Instrument Co., Ltd., Shanghai, China).

    TUNEL Assay:

    Article Title: Effect of Berberine from Coptis chinensis on Apoptosis of Intestinal Epithelial Cells in a Mouse Model of Ulcerative Colitis: Role of Endoplasmic Reticulum Stress
    Article Snippet: .. The chemicals utilized in this study were as follows: dextran sulfate sodium (DSS; MP Biomedicals, LLC, Solon, OH, USA; lot number: BJ14745, molecular weight: 5000); berberine hydrochloride (BBR; Shanghai Yuanye Bio-technology Co., Ltd., Shanghai, China; lot number: Y18D8C50814, molecular weight: 371.81); glucose-regulated protein 78 (GRP78), caspase-12, and caspase-3 primary antibodies (Abcam, Cambridge, UK; lot numbers: GR309483-1, ab62484, and ab13847, respectively); anti-rabbit and anti-mouse secondary antibody (Beijing ZSGB-BIO, Beijing, China; lot numbers: K167722B and K175212C, respectively); proteinase K (Beijing Tiangen, Beijing, China; lot number: M2011); DAB chromogenic reagent kit (Beijing ZSGB-BIO, lot number: K167722A); TUNEL in situ apoptosis detection kit (Roche, Basel, Switzerland; lot number: 11906800); high-purity total RNA rapid extraction kit (Shanghai Generay, Shanghai, China; lot number: 1703G01); HiScript-II Q RT SuperMix for qPCR reverse transcription kit (Nanjing Vazyme, Nanjing, China; lot number: 7E092G6); and ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme Co.; lot number: 7E092H6). .. The following instruments were used in this study: a RM2235 rotary microtome (Leica, Wetzlar, Germany); TEC-2500 histopathology dryer (Changzhou Hao Silin Instrument Equipment Co., Ltd., Nanjing, China); BX43 microscope (Olympus, Tokyo, Japan); PYX-DHS500BS-II water jacket constant-temperature incubator (Shanghai Yuejin Medical Instrument Co., Ltd.); BCD-211KD3 refrigerator (TCL, Huizhou, China); C21-SDHC15K induction heater (Zhejiang Shaoxing Supor Life Electric Co., Ltd., Hangzhou, China); and 101-3 electric heating constant-temperature dryer (Shanghai Jinping Instrument Co., Ltd., Shanghai, China).

    In Situ:

    Article Title: Effect of Berberine from Coptis chinensis on Apoptosis of Intestinal Epithelial Cells in a Mouse Model of Ulcerative Colitis: Role of Endoplasmic Reticulum Stress
    Article Snippet: .. The chemicals utilized in this study were as follows: dextran sulfate sodium (DSS; MP Biomedicals, LLC, Solon, OH, USA; lot number: BJ14745, molecular weight: 5000); berberine hydrochloride (BBR; Shanghai Yuanye Bio-technology Co., Ltd., Shanghai, China; lot number: Y18D8C50814, molecular weight: 371.81); glucose-regulated protein 78 (GRP78), caspase-12, and caspase-3 primary antibodies (Abcam, Cambridge, UK; lot numbers: GR309483-1, ab62484, and ab13847, respectively); anti-rabbit and anti-mouse secondary antibody (Beijing ZSGB-BIO, Beijing, China; lot numbers: K167722B and K175212C, respectively); proteinase K (Beijing Tiangen, Beijing, China; lot number: M2011); DAB chromogenic reagent kit (Beijing ZSGB-BIO, lot number: K167722A); TUNEL in situ apoptosis detection kit (Roche, Basel, Switzerland; lot number: 11906800); high-purity total RNA rapid extraction kit (Shanghai Generay, Shanghai, China; lot number: 1703G01); HiScript-II Q RT SuperMix for qPCR reverse transcription kit (Nanjing Vazyme, Nanjing, China; lot number: 7E092G6); and ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme Co.; lot number: 7E092H6). .. The following instruments were used in this study: a RM2235 rotary microtome (Leica, Wetzlar, Germany); TEC-2500 histopathology dryer (Changzhou Hao Silin Instrument Equipment Co., Ltd., Nanjing, China); BX43 microscope (Olympus, Tokyo, Japan); PYX-DHS500BS-II water jacket constant-temperature incubator (Shanghai Yuejin Medical Instrument Co., Ltd.); BCD-211KD3 refrigerator (TCL, Huizhou, China); C21-SDHC15K induction heater (Zhejiang Shaoxing Supor Life Electric Co., Ltd., Hangzhou, China); and 101-3 electric heating constant-temperature dryer (Shanghai Jinping Instrument Co., Ltd., Shanghai, China).

    Extraction:

    Article Title: Effect of Berberine from Coptis chinensis on Apoptosis of Intestinal Epithelial Cells in a Mouse Model of Ulcerative Colitis: Role of Endoplasmic Reticulum Stress
    Article Snippet: .. The chemicals utilized in this study were as follows: dextran sulfate sodium (DSS; MP Biomedicals, LLC, Solon, OH, USA; lot number: BJ14745, molecular weight: 5000); berberine hydrochloride (BBR; Shanghai Yuanye Bio-technology Co., Ltd., Shanghai, China; lot number: Y18D8C50814, molecular weight: 371.81); glucose-regulated protein 78 (GRP78), caspase-12, and caspase-3 primary antibodies (Abcam, Cambridge, UK; lot numbers: GR309483-1, ab62484, and ab13847, respectively); anti-rabbit and anti-mouse secondary antibody (Beijing ZSGB-BIO, Beijing, China; lot numbers: K167722B and K175212C, respectively); proteinase K (Beijing Tiangen, Beijing, China; lot number: M2011); DAB chromogenic reagent kit (Beijing ZSGB-BIO, lot number: K167722A); TUNEL in situ apoptosis detection kit (Roche, Basel, Switzerland; lot number: 11906800); high-purity total RNA rapid extraction kit (Shanghai Generay, Shanghai, China; lot number: 1703G01); HiScript-II Q RT SuperMix for qPCR reverse transcription kit (Nanjing Vazyme, Nanjing, China; lot number: 7E092G6); and ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme Co.; lot number: 7E092H6). .. The following instruments were used in this study: a RM2235 rotary microtome (Leica, Wetzlar, Germany); TEC-2500 histopathology dryer (Changzhou Hao Silin Instrument Equipment Co., Ltd., Nanjing, China); BX43 microscope (Olympus, Tokyo, Japan); PYX-DHS500BS-II water jacket constant-temperature incubator (Shanghai Yuejin Medical Instrument Co., Ltd.); BCD-211KD3 refrigerator (TCL, Huizhou, China); C21-SDHC15K induction heater (Zhejiang Shaoxing Supor Life Electric Co., Ltd., Hangzhou, China); and 101-3 electric heating constant-temperature dryer (Shanghai Jinping Instrument Co., Ltd., Shanghai, China).

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of Berberine from Coptis chinensis on Apoptosis of Intestinal Epithelial Cells in a Mouse Model of Ulcerative Colitis: Role of Endoplasmic Reticulum Stress
    Article Snippet: .. The chemicals utilized in this study were as follows: dextran sulfate sodium (DSS; MP Biomedicals, LLC, Solon, OH, USA; lot number: BJ14745, molecular weight: 5000); berberine hydrochloride (BBR; Shanghai Yuanye Bio-technology Co., Ltd., Shanghai, China; lot number: Y18D8C50814, molecular weight: 371.81); glucose-regulated protein 78 (GRP78), caspase-12, and caspase-3 primary antibodies (Abcam, Cambridge, UK; lot numbers: GR309483-1, ab62484, and ab13847, respectively); anti-rabbit and anti-mouse secondary antibody (Beijing ZSGB-BIO, Beijing, China; lot numbers: K167722B and K175212C, respectively); proteinase K (Beijing Tiangen, Beijing, China; lot number: M2011); DAB chromogenic reagent kit (Beijing ZSGB-BIO, lot number: K167722A); TUNEL in situ apoptosis detection kit (Roche, Basel, Switzerland; lot number: 11906800); high-purity total RNA rapid extraction kit (Shanghai Generay, Shanghai, China; lot number: 1703G01); HiScript-II Q RT SuperMix for qPCR reverse transcription kit (Nanjing Vazyme, Nanjing, China; lot number: 7E092G6); and ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme Co.; lot number: 7E092H6). .. The following instruments were used in this study: a RM2235 rotary microtome (Leica, Wetzlar, Germany); TEC-2500 histopathology dryer (Changzhou Hao Silin Instrument Equipment Co., Ltd., Nanjing, China); BX43 microscope (Olympus, Tokyo, Japan); PYX-DHS500BS-II water jacket constant-temperature incubator (Shanghai Yuejin Medical Instrument Co., Ltd.); BCD-211KD3 refrigerator (TCL, Huizhou, China); C21-SDHC15K induction heater (Zhejiang Shaoxing Supor Life Electric Co., Ltd., Hangzhou, China); and 101-3 electric heating constant-temperature dryer (Shanghai Jinping Instrument Co., Ltd., Shanghai, China).

    Reverse Transcription:

    Article Title: Effect of Berberine from Coptis chinensis on Apoptosis of Intestinal Epithelial Cells in a Mouse Model of Ulcerative Colitis: Role of Endoplasmic Reticulum Stress
    Article Snippet: .. The chemicals utilized in this study were as follows: dextran sulfate sodium (DSS; MP Biomedicals, LLC, Solon, OH, USA; lot number: BJ14745, molecular weight: 5000); berberine hydrochloride (BBR; Shanghai Yuanye Bio-technology Co., Ltd., Shanghai, China; lot number: Y18D8C50814, molecular weight: 371.81); glucose-regulated protein 78 (GRP78), caspase-12, and caspase-3 primary antibodies (Abcam, Cambridge, UK; lot numbers: GR309483-1, ab62484, and ab13847, respectively); anti-rabbit and anti-mouse secondary antibody (Beijing ZSGB-BIO, Beijing, China; lot numbers: K167722B and K175212C, respectively); proteinase K (Beijing Tiangen, Beijing, China; lot number: M2011); DAB chromogenic reagent kit (Beijing ZSGB-BIO, lot number: K167722A); TUNEL in situ apoptosis detection kit (Roche, Basel, Switzerland; lot number: 11906800); high-purity total RNA rapid extraction kit (Shanghai Generay, Shanghai, China; lot number: 1703G01); HiScript-II Q RT SuperMix for qPCR reverse transcription kit (Nanjing Vazyme, Nanjing, China; lot number: 7E092G6); and ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme Co.; lot number: 7E092H6). .. The following instruments were used in this study: a RM2235 rotary microtome (Leica, Wetzlar, Germany); TEC-2500 histopathology dryer (Changzhou Hao Silin Instrument Equipment Co., Ltd., Nanjing, China); BX43 microscope (Olympus, Tokyo, Japan); PYX-DHS500BS-II water jacket constant-temperature incubator (Shanghai Yuejin Medical Instrument Co., Ltd.); BCD-211KD3 refrigerator (TCL, Huizhou, China); C21-SDHC15K induction heater (Zhejiang Shaoxing Supor Life Electric Co., Ltd., Hangzhou, China); and 101-3 electric heating constant-temperature dryer (Shanghai Jinping Instrument Co., Ltd., Shanghai, China).



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